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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP2A All Species: 6.36
Human Site: T1322 Identified Species: 10
UniProt: P11388 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11388 NP_001058.2 1531 174385 T1322 T E P R R A A T K T K F T M D
Chimpanzee Pan troglodytes XP_516332 1634 184611 R1369 I P R D S L L R R A A A E R P
Rhesus Macaque Macaca mulatta XP_001092092 1620 182490 R1355 I P R D S L L R R A A A E R P
Dog Lupus familis XP_537646 1532 174610 K1323 E P R R A A T K T K F T V D L
Cat Felis silvestris
Mouse Mus musculus Q01320 1528 172859 A1318 K E Q R S R A A K A K F T V D
Rat Rattus norvegicus P41516 1526 173202 T1318 K E P R I A A T K A K F T A D
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001519680 1746 195650 A1352 T K P R R A A A K S K Y T V D
Chicken Gallus gallus O42130 1553 174974 D1344 D L T S S D E D S E Y Q E N S
Frog Xenopus laevis NP_001082502 1579 178601 S1337 A K A N Y I L S S D E E S A K
Zebra Danio Brachydanio rerio NP_001003834 1574 177254 V1331 K R E R R A A V E K K Y S L S
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P15348 1447 164377 E1238 P V E F K I T E E I I K K M A
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q23670 1520 172316 E1307 K E P K E P K E P K K V K K E
Sea Urchin Strong. purpuratus XP_783546 1448 163750 L1239 T K P R K Q K L L T R E E T K
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30182 1473 164089 S1256 A K K K A S E S E T T E A S H
Baker's Yeast Sacchar. cerevisiae P06786 1428 164196 K1220 A R K G K K I K L E D K N F E
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 67.2 67.5 93.6 N.A. 89 88.7 N.A. 71.3 76.3 70.7 67.9 N.A. 51.2 N.A. 53 58.9
Protein Similarity: 100 78.6 78.4 96.4 N.A. 93.6 93.2 N.A. 79.1 85 81.8 78.8 N.A. 66.8 N.A. 70.4 73.4
P-Site Identity: 100 0 0 13.3 N.A. 53.3 73.3 N.A. 66.6 0 0 33.3 N.A. 6.6 N.A. 20 26.6
P-Site Similarity: 100 6.6 6.6 13.3 N.A. 60 73.3 N.A. 93.3 0 26.6 60 N.A. 20 N.A. 33.3 46.6
Percent
Protein Identity: N.A. N.A. N.A. 45 42.2 N.A.
Protein Similarity: N.A. N.A. N.A. 61.4 60.4 N.A.
P-Site Identity: N.A. N.A. N.A. 6.6 0 N.A.
P-Site Similarity: N.A. N.A. N.A. 40 13.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 20 0 7 0 14 34 34 14 0 27 14 14 7 14 7 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 7 0 0 14 0 7 0 7 0 7 7 0 0 7 27 % D
% Glu: 7 27 14 0 7 0 14 14 20 14 7 20 27 0 14 % E
% Phe: 0 0 0 7 0 0 0 0 0 0 7 20 0 7 0 % F
% Gly: 0 0 0 7 0 0 0 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 % H
% Ile: 14 0 0 0 7 14 7 0 0 7 7 0 0 0 0 % I
% Lys: 27 27 14 14 20 7 14 14 27 20 40 14 14 7 14 % K
% Leu: 0 7 0 0 0 14 20 7 14 0 0 0 0 7 7 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 14 0 % M
% Asn: 0 0 0 7 0 0 0 0 0 0 0 0 7 7 0 % N
% Pro: 7 20 34 0 0 7 0 0 7 0 0 0 0 0 14 % P
% Gln: 0 0 7 0 0 7 0 0 0 0 0 7 0 0 0 % Q
% Arg: 0 14 20 47 20 7 0 14 14 0 7 0 0 14 0 % R
% Ser: 0 0 0 7 27 7 0 14 14 7 0 0 14 7 14 % S
% Thr: 20 0 7 0 0 0 14 14 7 20 7 7 27 7 0 % T
% Val: 0 7 0 0 0 0 0 7 0 0 0 7 7 14 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 7 0 0 0 0 0 7 14 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _